CLUSTAL W (1.82) Multiple Sequence Alignments



Sequence format is Pearson
Sequence 1: 1HOW_A          373 aa
Sequence 2: 1CSN__          298 aa
Sequence 3: 1DM2_A          298 aa
Sequence 4: 1KOA__          491 aa
Sequence 5: 2CPK_E          350 aa
Sequence 6: 1JST_B          258 aa
Start of Pairwise alignments
Aligning...
Sequences (1:2) Aligned. Score:  17
Sequences (1:3) Aligned. Score:  29
Sequences (1:4) Aligned. Score:  17
Sequences (1:5) Aligned. Score:  19
Sequences (1:6) Aligned. Score:  8
Sequences (2:3) Aligned. Score:  10
Sequences (2:4) Aligned. Score:  18
Sequences (2:5) Aligned. Score:  16
Sequences (2:6) Aligned. Score:  5
Sequences (3:4) Aligned. Score:  23
Sequences (3:5) Aligned. Score:  23
Sequences (3:6) Aligned. Score:  11
Sequences (4:5) Aligned. Score:  23
Sequences (4:6) Aligned. Score:  3
Sequences (5:6) Aligned. Score:  6
Guide tree        file created:   [/ebi/extserv/old-work/845993.703181-26819.dnd]
Start of Multiple Alignment
There are 5 groups
Aligning...
Group 1:                     Delayed
Group 2:                     Delayed
Group 3:                     Delayed
Group 4:                     Delayed
Group 5:                     Delayed
Sequence:3     Score:3067
Sequence:4     Score:2531
Sequence:5     Score:2501
Sequence:2     Score:2117
Sequence:6     Score:1662
Alignment Score 416
CLUSTAL-Alignment file created  [/ebi/extserv/old-work/845993.703181-26819.aln]

CLUSTAL W (1.82) multiple sequence alignment


1HOW_A          --------------------------------DYRPGGFHPAFKGEPYKDARYILVRKLG 28
1DM2_A          -------------------------------------------------MENFQKVEKIG 11
1KOA__          RKRRRGYDVDEQGKIVRGKGTVSSNYDNYVFDIWKQYYPQPVEIKHDHVLDHYDIHEELG 60
2CPK_E          --------GNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQ--NTAQLDQFDRIKTLG 50
1CSN__          -----------------------------------------MSGQNNVVGVHYKVGRRIG 19
1JST_B          --------------------------------------------VPDYHEDIHTYLREME 16
                                                        .           .   . : 

1HOW_A          WGHFSTVWLAKDMVNNTHVAMKIV-RGDKVYTEAAEDEIKLLQRVNDADNTKEDSMGANH 87
1DM2_A          EGTYGVVYKARNKLTGEVVALKKI-RLDTETEGVPSTAIREISLLKELNHPN-------- 62
1KOA__          TGAFGVVHRVTERATGNNFAAKFV-MTP--HESDKETVRKEIQTMSVLRHPT-------- 109
2CPK_E          TGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPF-------- 102
1CSN__          EGSFGVIFEGTNLLNNQQVAIKFE-----PRRSDAPQLRDEYRTYKLLAGCTG------- 67
1JST_B          VKCKPKVGYMKKQPDITNSMRAIL--VDWLVEVGEEYKLQNETLHLAVNYIDR------- 67
                      :    .                                         .: .:. 

1HOW_A          ILKLLDHFNHKGPNGVHVVMVFEVLGENLLALIKKYEHRGIPLIYVKQISKQLLLGLDYM 147
1DM2_A          IVKLLDVIHTEN----KLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFC 118
1KOA__          LVNLHDAFEDDN----EMVMIYEFMSGGELFEKVADEHNKMSEDEAVEYMRQVCKGLCHM 165
2CPK_E          LVKLEFSFKDNS----NLYMVMEYVAGGEMFSHLR-RIGRFSEPHARFYAAQIVLTFEYL 157
1CSN__          -IPNVYYFGQEG---LHNVLVIDLLGPS-LEDLLDLCGRKFSVKTVAMAAKQMLARVQSI 122
1JST_B          FLSSMSVLRGKL----QLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHL 123
                 :     :  . ... .       :  .            :          :    .   

1HOW_A          HRRCGIIHTDIKPENVLMEIVDSPENLIQIKIADLGNACWY----------DEHYTNSIQ 197
1DM2_A          HSHR-VLHRDLKPQNLLINTEG------AIKLADFGLARAFGV-------PVRTYTHEVV 164
1KOA__          HENN-YVHLDLKPENIMFTTKRSN----ELKLIDFGLTAHLD--------PKQSVKVTTG 212
2CPK_E          HSLD-LIYRDLKPENLLIDQQG------YIQVTDFGFAKRVK----------GRTWTLCG 200
1CSN__          HEKS-LVYRDIKPDNFLIGRPNSKNAN-MIYVVDFGMVKFYRDPVTKQHIPYREKKNLSG 180
1JST_B          VLKVLTFDLAAPTVNQFL----------TQYFLHQQPAN---------------CKVESL 158
                      .     . * ::    : .      . .   .                      

1HOW_A          TREYRSPEVLLGAPWG-CGADIWSTACLIFELITGDFLFEPDEGHSYTK----------- 245
1DM2_A          TLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDS----------------- 207
1KOA__          TAEFAAPEVAEGKPVG-YYTDMWSVGVLSYILLSGLSPFGGENDDETLRNVKSCDWNMDD 271
2CPK_E          TPEYLAPEIILSKGYN-KAVDWWALGVLIYEMAAGYPPFFADQP---------------- 243
1CSN__          TARYMSINTHLGREQS-RRDDLEALGHVFMYFLRGSLPWQGLKAATN------------- 226
1JST_B          AMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPES----------------- 201
                :      .   .        .  : . .   :          .                 

1HOW_A          --------------------DDDHIAQIIELLGELPSYLLRNGKYTRTFFNSR-GLLRNI 284
1DM2_A          --------------------EIDQLFRIFRTLGTPDEVVWPG---VTSMPDYK-PSFPKW 243
1KOA__          SAFSGISEDGKDFIRKLLLADPNTRMTIHQALEHPWLTPGNAPGRDSQIPSSRYTKIRDS 331
2CPK_E          -------------------------IQIYEKIVSGKVRFPSH--FSSDLKDLLRNLLQVD 276
1CSN__          -------------------------KQKYERIGEKKQSTPLR-ELCAGFPEEFYKYMHYA 260
1JST_B          ---------------------------LIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQS 234
                                    . .      .                              

1HOW_A          SKLKFWPLEDVLTEKYKFSKDEAKEISDFLSPMLQLDPRKRADAGGLVNHPWLKDTLGME 344
1DM2_A          ARQDFSKVVPPLDEDGRS----------LLSQMLHYDPNKRISAKAALAHPFFQDVT--- 290
1KOA__          IKTKYDAWPEPLPPLGRISNYSSLRKHRPQEYSIRDAFWDRSEAQPRFIVKPYGTEVGEG 391
2CPK_E          LTKRFGNLKNGVNDIKNHK--------WFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFD 328
1CSN__          RNLAFDATPD--------------------YDYLQGLFSKVLERLNTTEDENFDWNLL-- 298
1JST_B          IREKYK--------------------------------NSKYHGVSLLNPPETLNL---- 258
                    :             .. .:                                     

1HOW_A          EIRVPDRELYGSGSDIPGWFEEVRDHKRH------------------------------- 373
1DM2_A          -KPVPHLRL--------------------------------------------------- 298
1KOA__          QSANFYCRVIASSPPVVTWHKDDRELKQSVKYMKRYNGNDYGLTINRVKGDDKGEYTVRA 451
2CPK_E          DYEEEEIRVSINEKCGKEFTEF-------------------------------------- 350
1CSN__          ------------------------------------------------------------
1JST_B          ------------------------------------------------------------
                .          .        .   . .                                 

1HOW_A          ----------------------------------------
1DM2_A          ----------------------------------------
1KOA__          KNSYGTKEEIVFLNVTRHSEPLKFEPLEPMKKAPSPPRVE 491
2CPK_E          ----------------------------------------
1CSN__          ----------------------------------------
1JST_B          ----------------------------------------
                                                        

(
(
(
(
1HOW_A:0.36167,
1DM2_A:0.34639)
:0.02821,
1JST_B:0.51699)
:0.02324,
1CSN__:0.42955)
:0.01502,
1KOA__:0.38503,
2CPK_E:0.38354);