1F5S: Phosphoserine Phosphatase
Sequence Alignment
Sequence alignment was done by using Clustalw. Somehow, I wasn’t able to get the 1FQU
comparison, so I only aligned the other three.
Below are the results:
CLUSTAL W (1.82) Multiple Sequence Alignments
Sequence format is Pearson
Sequence 1: 1EUL_A 994 aa
Sequence 2: 1J97_A 211 aa
Sequence 3: 1F5S_A 211 aa
Start of Pairwise alignments
Aligning...
Sequences (1:2) Aligned. Score: 22
Sequences (1:3) Aligned. Score: 22
Sequences (2:3) Aligned. Score: 99
Guide tree file created: [/ebi/extserv/old-work/995948.773247.dnd]
Start of Multiple Alignment
There are 2 groups
Aligning...
Group 1: Sequences: 2 Score:4480
Group 2: Delayed
Sequence:1 Score:1678
Alignment Score 1405
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A MEAAHSKSTEECLAYFGVSETTGLTPDQVKRHLEKYGHNELPAEEGKSLWELVIEQFEDL 60
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A LVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALK 120
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A EYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILSIKSTTLRVDQSIL 180
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A TGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVSTEIGKIRDQMA 240
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A ATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWIRGAIYYFKIAV 300
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A ALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQ 360
1F5S_A MEKKKKLILFDFDSTLVNNETIDEIAREAGVEEEVKKITKEAMEGKLNFEQSLRKRVSLL 60
1J97_A MEKKKKLILFXFDSTLVNNETIDEIAREAGVEEEVKKITKEAMEGKLNFEQSLRKRVSLL 60
1EUL_A MSVCKMFIIDKVDGDFCSLNEFSITGSTYAPEGEVLKNDKPIRSGQFDGLVELATICALC 420
*. * :*: .*. : . : :. . . * ** * * .*::: .* . :*
1F5S_A KDLPIEKVEKA------------------------------IKRITPTEGAEETIKELKN 90
1J97_A KDLPIEKVEKA------------------------------IKRITPTEGAEETIKELKN 90
1EUL_A NDSSLDFNETKGVYEKVGEATETALTTLVEKMNVFNTEVRNLSKVERANACNSVIRQLMK 480
:* .:: *. :.:: ::..:..*::* :
1F5S_A RGYVVAVVSGGFDIAVNKIKEKLGLDYAFANRLIVKDG------------------KLTG 132
1J97_A RGYVVAVVSGGFDIAVNKIKEKLGLDYAFANRLIVKDG------------------KLTG 132
1EUL_A KEFTLEFSRDRKSMSVYCSPAKS-SRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPMTG 539
: :.: . . .::* * *..*:::**.. :**
1F5S_A DVEG---EVLKENAKG---------EILEKIAKIEGINLED------------------- 161
1J97_A DVEG---EVLKENAKG---------EILEKIAKIEGINLED------------------- 161
1EUL_A PVKEKILSVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSSRFMEYETDLTFVGVVGM 599
*: .*:** ..* :. .* * : *:*
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A LDPPRKEVMGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFD 659
1F5S_A --------------------------------------TVAVGDGANDISMFKKAGLKIA 183
1J97_A --------------------------------------TVAVGDGANDISMFKKAGLKIA 183
1EUL_A DLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIA 719
*. .***.** . :*** : **
1F5S_A FCAKPILKEKADICIEKRD------------------LREILKYIK-------------- 211
1J97_A FCAKPILKEKADICIEKRD------------------LREILKYIK-------------- 211
1EUL_A MGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTA 779
: : . : :.*. : * ::::::*:
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A ALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMA 839
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A IGGYVGAATVGAAAWWFMYAEDGPGVTYHQLTHFMQCTEDHPHFEGLDCEIFEAPEPMTM 899
1F5S_A ------------------------------------------------------------
1J97_A ------------------------------------------------------------
1EUL_A ALSVLVTIEMCNALNSLSENQSLMRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKL 959
1F5S_A -----------------------------------
1J97_A -----------------------------------
1EUL_A KALDLTQWLMVLKISLPVIGLDEILKFIARNYLEG 994